The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR merchandise were being digested and cloned into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. A point mutation was introduced by the https://andersonp887bmw9.wikinewspaper.com/user